|
KMID : 0545120180280111865
|
|
Journal of Microbiology and Biotechnology
2018 Volume.28 No. 11 p.1865 ~ p.1875
|
|
|
Application and Analysis of Rhizopus oryzae Mycelia Extending Characteristic in Solid-state Fermentation for Producing Glucoamylase
|
|
Tang Xianghua
Luo Tianbao
Li Xue
Yang Huanhuan
Yang Yunjuan
Li Junjun
Xu Bo
Huang Zunxi
|
|
|
Abstract
|
|
|
|
Enhanced application of solid-state fermentation (SSF) in industrial production and the influence of SSF of Rhizopus K1 on glucoamylase productivity were analyzed using the flat band method. A growth model was implemented through SSF of Rhizopus K1 in this experiment, and spectrophotometric method was used to determine glucoamylase activity. Results showed that in bran and potato culture medium with 70% moisture in a loose state, μ of mycelium reached to 0.15 h?1 after 45 h of culture in a thermostatic water bath incubator at 30°C. Under a low-magnification microscope, mycelial cells appeared uniform, bulky with numerous branches, and were not easily ruptured. The generated glucoamylase activity reached to 55 U/g (dry basis). This study has good utilization value for glucoamylase production by Rhizopus in SSF.
|
|
|
KEYWORD
|
|
|
Solid-state fermentation, growth model, Rhizopus oryzae, glucoamylase, hyphal extension, mycelial cells
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|